Double antibody pairs sandwich-ELISA (DAPS-ELISA) detects Acidovorax citrulli serotypes with broad coverage

Definition of three serotypes of Hantaviruses by a double sandwich ELISA with biotin-avidin amplification system.

A total of seven different isolates of Hantaviruses from five mammalian species and six geographical areas were examined by a double sandwich ELISA with biotin-avidin amplification system. Three serotypes were recognized by comparing antigen titres to homologous and heterologous antibody and blocking antibody titres to homologous and heterologous antigens. Serotype 1 included four strains: Hant...

Development and application of a double-antigen sandwich ELISA

A Double Antibody Sandwich ELISA Assay for the Detection of Salmonella in Food Original Samples

Development of monoclonal antibody based sandwich ELISA for rapid detection of Salmonella and optimization of enrichment procedures to increase the sensitivity. Spleen cells from Balb/c mice immunized with flagellin (H=d) antigen of S. typhi were fused with NSO myeloma clones for preparation of mAbs and New Zealand rabbits were used to produce polyclonal antibody. Different enrichment broths we...

Development of a double-antibody sandwich ELISA for rapid detection of Bacillus Cereus in food

Bacillus cereus is increasingly recognized as one of the major causes of food poisoning in the industrialized world. In this paper, we describe a sensitive double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) that was developed for rapid detection of B. cereus in food to minimize the risk of contamination. The polyclonal antibody (pAb) and monoclonal antibodies (mAbs) specific to ...

Preparation and characterization of anti-GP73 monoclonal antibodies and development of double-antibody sandwich ELISA.

BACKGROUND Serum Golgi protein 73 (GP73) as a novel and potential marker for diagnosing hepatocellular carcinoma (HCC) have been found to be elevated in HCC patients and associated with clinical variables representing tumor growth and invasiveness. The aim of this study was to prepare a pair of monoclonal antibodys (mAbs) against GP73 and develop a newly designed double-antibody sandwich enzyme...